Molecular Docking Assisted Isolation of Azadirachtin-A, from Seeds of Azadirachta indica Extract against Cervical Cancer

Abstract

Objectives: To isolate Azadirachtin-A, from Seeds of Azadirachta indica and to perform mol. docking studies for antiproliferative activity against cervical cancer. Methods: The Current studies on Azadirachtin-A, a potent cervical anticancer agent, was designed for the isolation of Azadirachtin-A, from seem fruits collected from local area (Ibrahimpatnam), the seeds were first separated, dried and made in to coarse powder. The seed kernels was defatted and extracted with methanol using soxhlet apparatus Methanolic extract of seeds were subjected to direct reverse phase High Performance Liquid Chromatog. (HPLC) for separation of Azadirachtin-A, by mol. docking of Azadirachtin-A, using Mol. Operating Environment (MOE) 2008 software Findings: This revealed that Azadirachtin-A, has an affinity of 59.2% and 72% towards amino acids like Glycine 131 and Lysine 89 with polar interactions. Azadirachtin-A, found to interact with Cyclin E, was retrieved from the Protein Data Bank (PDB code: 1W98). The Azadirachtin-A, showed excellent, Increase Life Span (63.44 and 82.56), reduced the viable cells (30.4±1.5 and 24.5±1.6), and tumor volume (1.2±0.05 and 1.0±0.05) when compared to standard drug 5-Fluorouracil. Application: Azadirachtin-A, showed significant docking interaction with Cyclin E, active site using windows 2002, MOE 2008 software. Result showed that Azadirachtin-A, may lead to potent cervical anticancer agent.