Genes to Genomics: Mapping the Fungal Genome

Abstract

Although we discussed molecular markers in the Chapter 7, and thereby leaned toward physical analysis, the theme of our story so far has been the use of the classic approach of genetics: making an organized series of crosses and using gene segregations to deduce chromosome maps. Entirely conventional procedures can result in those chromosomal maps showing the location of genes and centromeres. Then, by using molecular markers, the telomeres of the chromosome, and as many other loci as we are willing to characterize and include in our crosses, can be mapped along the chromosomes of an organism. Large-scale, high-density linkage maps can be assembled in this way. A high-density linkage map is conventionally considered to be one with one gene or marker for each 1% recombination in the genome.