Nucleotide Composition Analysis: Use in Metagenome Analysis

Abstract

SynonymsBinning; Genome signature; Nucleotide frequencyDefinitionThe composition of nucleotide bases in a microbial genome is not random and is instead biased toward different compositional structures that vary between organisms. These biases occur as identifiable patterns in oligonucleotide base composition, and it is by these patterns that otherwise anonymous metagenomic sequences are grouped into inferred populations. This allows for more in-depth analysis of the functional potential of a sampled microbial community in the context of constituent members (inferred populations).IntroductionThe composition of nucleotide bases in a microbial genome is not random and is instead biased toward different compositional structures that vary between organisms. These biases occur as identifiable patterns in oligonucleotide base composition, and it is by these patterns that otherwise anonymous metagenomic sequences can be grouped into inferred populations enabling in-depth functional analysis. ...