A new approach for small ruminant lentivirus full genome characterization revealed the circulation of divergent strains

28Citations
Citations of this article
36Readers
Mendeley users who have this article in their library.

Abstract

Small Ruminant Lentiviruses (SRLV) include at least 4 viral highly divergent genotypes. Genotypes A and B are widely distributed and genotypes C and E have been recognized in restricted geographic areas. New phylogroups have been identified targeting conserved regions. However, this approach suffers from the potential risk to misamplify highly divergent strains. Pathogenic strains are easily adapted to fibroblastic cells, but non-pathogenic strains isolation may require a different approach. We developed a fast and effective method for SRLV full genome characterization after cell culture isolation. Spleen samples were collected during regular slaughter from sheep and goats in northwestern Italy. Spleen-derived macrophage cultures were monitored for reverse transcriptase activity and RNA was extracted from the supernatant of positive cultures. Using Illumina MiSeq platform 22 new full genome sequences were obtained. The success of this approach is based on the following features: spleen is one of the main target for SRLV persistence; red pulp is a reserve of resident macrophages, the main target for SRLV replication in vivo; RTA is a sensitive assay for any replicating retrovirus; de novo sequencing do not require genetic knowledge in advance.

Cite

CITATION STYLE

APA

Colitti, B., Coradduzza, E., Puggioni, G., Capucchio, M. T., Reina, R., Bertolotti, L., & Rosati, S. (2019). A new approach for small ruminant lentivirus full genome characterization revealed the circulation of divergent strains. PLoS ONE, 14(2). https://doi.org/10.1371/journal.pone.0212585

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free