Mapping of HIV-1 determinants of apoptosis in infected T cells

17Citations
Citations of this article
10Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

HIV-1 infection leads to death of CD4+ T cells in vivo and in vitro, although the mechanisms of this cell death are not well defined. We used flow cytometry to concurrently analyze infection and apoptosis of the CD4+ CEM T cell line and human peripheral blood mononuclear cells (PBMC). Surprisingly, T cells productively infected with HIV-1 IIIB showed less apoptosis than control, uninfected T cells. This relative paucity of apoptosis was a characteristic of IIIB, since a large number of cells infected with the viral clone, HIV-1 NL4-3, were apoptotic. The nef, vpr, and vpu gene products were not responsible for apoptosis of NL4-3-infected cells, since NL4- 3ΔVprΔVpuΔNef and HXB-2 (a nef, vpr, and vpu triple mutant derived from IIIB) also killed infected cells. Moreover, only IIIB-infected cells showed a resistance to background levels of apoptosis. Thus, the apoptotic (and antiapoptotic) properties of HIV-1 do not map solely to mutations in nef, vpr, or vpu. We postulate that, in vivo, HIV variants that do not induce rapid apoptosis in the cells they infect may have a selective advantage.

Cite

CITATION STYLE

APA

Rapaport, E., Casella, C. R., Iklé, D., Mustafa, F., Isaak, D., & Finkel, T. H. (1998). Mapping of HIV-1 determinants of apoptosis in infected T cells. Virology, 252(2), 407–417. https://doi.org/10.1006/viro.1998.9459

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free