Antibiotic-free, efficient in vitro selection in plant genetic engineering can improve risk perception and speed up pre-market scrutiny of genetically modified crops. We provide a protocol for genetic transformation of two important crops, durum wheat and alfalfa, using a bacterial and a plant-derived selectable marker gene encoding mutated, gabaculine-insensitive glutamate 1-semialdehyde aminotransferase (GSA) enzymes. These methods can potentially be applied, with minor adaptations, to many other monocot and dicot crop plants.
CITATION STYLE
Ferradini, N., Giancaspro, A., Nicolia, A., Gadaleta, A., Veronesi, F., & Rosellini, D. (2016). Efficient, antibiotic marker-free transformation of a dicot and a monocot crop with glutamate 1-semialdehyde aminotransferase selectable marker genes. In Methods in Molecular Biology (Vol. 1385, pp. 89–98). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3289-4_6
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