Caveolae are plasma membrane organelles that are, among many other features, involved in mechanosensing and mechanoprotection. Different tools have been developed to study caveolae-dependent mechanoprotection and had to be adapted to the tissue or cells studied, as these structures are found in almost every type of cells. This chapter focuses on a protocol combining the use of live-cell imaging, micropatterning, hypo-osmotic shock as a mechanical stress, and dyes such as calcein-AM and propidium iodide. We used this protocol for the in vitro study of the effect of mechanical stress on membrane integrity in human muscle cells from patients bearing caveolin-3 mutations.
CITATION STYLE
Dewulf, M., & Blouin, C. M. (2020). Study of caveolae-dependent mechanoprotection in human muscle cells using micropatterning and live-cell microscopy. In Methods in Molecular Biology (Vol. 2169, pp. 189–196). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0732-9_17
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