Luciferase reporter assays are widely used to study promoter activity, transcription factors, intracellular signaling, protein interactions (Jia et al., PloS One 6:e26414), miRNA processing (Allegra and Mertens, Biochem Biophys Res Commun 406:501-505), and target recognition (Jin et al., Methods Mol Biol 936:117-127). Here we describe the use of a dual-luciferase reporter system to evaluate the enzymatic activity of a key enzyme involved in RNA maturation-DROSHA. This dual system is a simple and fast method for the quantification of the DROSHA processing activity in live cells. © 2014 Springer Science+Business Media, New York.
CITATION STYLE
Bilan, V., Allegra, D., Kuchenbauer, F., & Mertens, D. (2014). In vivo processing assay based on a dual-luciferase reporter system to evaluate DROSHA enzymatic activity. Methods in Molecular Biology, 1095, 87–93. https://doi.org/10.1007/978-1-62703-703-7_6
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