Membrane protein reconstitution in liposomes is an invaluable technique to study numerous properties of membrane proteins in vitro. Kinetics, substrate specificity, and protein-protein interaction of membrane proteins can be investigated once they are embedded in the liposome bilayer. Both protocols described here aim to investigate the efflux pump MexA-MexB-OprM from Pseudomonas aeruginosa, a proteins complex embedded in both membranes of this Gram-negative bacteria. This tri-partite system, which by-passes the periplams, is involved in antibiotic resistance. First, we describe a protocol to study MexB, the actual transporter, and second we propose an alternate protocol where the tripartite system is investigated as a whole.
CITATION STYLE
Verchère, A., Broutin, I., & Picard, M. (2017). Reconstitution of membrane proteins in liposomes. In Methods in Molecular Biology (Vol. 1635, pp. 259–282). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7151-0_14
Mendeley helps you to discover research relevant for your work.