Background: The vesicular B0AT3 transporter (SLC6A17), one of the members of the SLC6 family, is a transporter for neutral amino acids and is exclusively expressed in brain. Here we provide a comprehensive expression profile of B0AT3 in mouse brain using in situ hybridization and immunohistochemistry.Results: We confirmed previous expression data from rat brain and used a novel custom made antibody to obtain detailed co-labelling with several cell type specific markers. B0AT3 was highly expressed in both inhibitory and excitatory neurons. The B0AT3 expression was highly overlapping with those of vesicular glutamate transporter 2 (VGLUT2) and vesicular glutamate transporter 1 (VGLUT1). We also show here that Slc6a17mRNA is up-regulated in animals subjected to short term food deprivation as well as animals treated with the serotonin reuptake inhibitor fluoxetine and the dopamine/noradrenaline reuptake inhibitor bupropion.Conclusions: This suggests that the B0AT3 transporter have a role in regulation of monoaminergic as well as glutamatergic synapses. © 2013 Hägglund et al.; licensee BioMed Central Ltd.
CITATION STYLE
Hägglund, M. G. A., Hellsten, S. V., Bagchi, S., Ljungdahl, A., Nilsson, V. C. O., Winnergren, S., … Fredriksson, R. (2013). Characterization of the transporterB0AT3 (Slc6a17) in the rodent central nervous system. BMC Neuroscience, 14. https://doi.org/10.1186/1471-2202-14-54
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