Raman Microscopy and Imaging: Applications to Skin Pharmacology and Wound Healing

Abstract

The utility of confocal Raman microscopy to study biological events in skin is demonstrated with three examples. (i) monitoring the spatial and structural differences between native and cultured skin, (ii) tracking the permeation and biochemical transformation in skin of a Vitamin E derivative and (iii) tracking the spatial distribution of three major skin proteins (keratin; collagen, and elastin) during wound healing in an explant skin model.