The pursuit of more potent, safe, and cost-effective drugs has placed a greater emphasis on antibody optimisation within the drug discovery process. Technologies to rapidly improve antibody drug performance, such as phage display, ribosome display, and yeast display, are playing a key role in this effort. Among these ribosome display is a particularly powerful technology and has recently been applied to the affinity optimisation of a humanised anti-receptor for advanced glycation end products (anti-RAGE) antibody (Finlay et al., J Mol Biol 388:541-558, 2009). By using a combination of error-prone PCR with ribosome display each amino acid position within this humanised antibody was scanned for both its functional importance and its capacity to increase affinity resulting in both affinity-matured antibody variants and a functional map of the antibody paratope. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Hufton, S. E. (2012). Affinity maturation and functional dissection of a humanised anti-rage monoclonal antibody by ribosome display. Methods in Molecular Biology, 805, 403–422. https://doi.org/10.1007/978-1-61779-379-0_23
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