Two different methods, stimulation of transport by fatty acyl-coenzyme A (CoA) and inhibition of transport by a nonhydrolyzable analogue of palmitoyl-CoA, reveal that fatty acylation is required to promote fusion of transport vesicles with Golgi cisternae. Specifically, fatty acyl-CoA is needed after the attachment of coated vesicles and subsequent uncoating of the vesicles, and after the binding of the NEM-sensitive fusion protein (NSF) to the membranes, but before the actual fusion event. We therefore suggest that an acylated transport component participates, directly or indirectly, in membrane fusion.
CITATION STYLE
Pfanner, N., Glick, B. S., Arden, S. R., & Rothman, J. E. (1990). Fatty acylation promotes fusion of transport vesicles with Golgi cisternae. Journal of Cell Biology, 110(4), 955–961. https://doi.org/10.1083/jcb.110.4.955
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