Using RNA-seq technology, we found that the majority of microRNAs (miRNAs) present in CFU-E erythroid progenitors are down-regulated during terminal erythroid differentiation. Of the developmentally down-regulated miRNAs, ectopic overexpression of miR-191 blocks erythroid enucleation but has minor effects on proliferation and differentiation. We identified two erythroid-enriched and developmentally up-regulated genes, Riok3 and Mxi1, as direct targets of miR-191. Knockdown of either Riok3 or Mxi1 blocks enucleation, and either physiological overexpression of miR-191 or knockdown of Riok3 or Mxi1 blocks chromatin condensation. Thus, downregulation of miR-191 is essential for erythroid chromatin condensation and enucleation by allowing up-regulation of Riok3 and Mxi1. © 2011 by Cold Spring Harbor Laboratory Press.
CITATION STYLE
Zhang, L., Flygare, J., Wong, P., Lim, B., & Lodish, H. F. (2011). miR-191 regulates mouse erythroblast enucleation by down-regulating Riok3 and Mxi1. Genes and Development, 25(2), 119–124. https://doi.org/10.1101/gad.1998711
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