The following chapter describes the purification of whole IgG by Protein A affinity chromatography from crude protein mixtures such as serum, ascites fluids or cell culture supernatant. This reliable method is based on the ability of Protein A to bind with high affinity to the Fc region of immunoblobulins.
CITATION STYLE
Zettlitz, K. A. (2010). Protein A/G Chromatography. In Antibody Engineering (pp. 531–535). Springer Berlin Heidelberg. https://doi.org/10.1007/978-3-642-01144-3_34
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