One of the main consequences of glucose action on pancreatic β-cells is the stimulation of Ca2+ entry as well as Ca2+ release from intracellular compartments. Therefore, one of the cornerstones of any diabetes research laboratory should be the ability to measure changes in intracellular calcium concentrations within pancreatic β-cells. There are a variety of methods available for the measurement of intracellular calcium, from multiple regions of interest (ROIs) within single cells to observe oscillating calcium, to population-based 96-well applications to enable high-throughput screening of the effects of novel agonists. These methods allow calcium signaling to be observed in a single cellular assay to look at oscillations at a cellular level, to view a population response, and to enable high-throughput assays where the mean reflects a single cell response.
CITATION STYLE
Selway, J. L. (2020). Measurement of calcium signaling in beta-cell lines using epifluorescence and confocal microscopy. In Methods in Molecular Biology (Vol. 2076, pp. 231–239). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9882-1_13
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