Simultaneous determination of seven flavonoids in Potentilla multifida by HPLC

Abstract

A reversed-phase high-performance liquid chromatographic method is described for the simultaneous determination of seven flavonoids in Potentilla multifida: hyperin, quercetin-3-O -β-D-glucopyranoside, luteolin-7-O -β-D-glucuronide, apigenin-7-O -β-D-glucuronide, quercetin, tribuloside, and apigenin. The method involves the use of a Hypersil octadecylsilyl silica (ODS) analytical column (125Å, 5 μm, 4.6 x 250 mm) at 25°C with the mixture of acetonitrile and aqueous H 3PO4 as the mobile phase and detection at 254 nm. The recovery of the method is 95.4-104.8%, and linearity (r> 0.9998) is obtained for all the flavonoids. The results indicate that the flavonoid content of P.multifida varied significantly from locality to locality.