Yeast as a Versatile System for Heterologous Protein Expression; Genetically Modified Yeast Strain to Express Human Drug Metabolism Enzymes

Abstract

A wide variety of recombinant expression systems are available today to produce large amounts of recombinant proteins. Choosing the right expression system for a particular application is the key to success, whether it is a prokaryotic system, viral system, systems mimicking eukaryotic expression systems like yeast, insect or mammalian cell line expression system. Model organisms are often chosen on the basis that they are amenable to experimental manipulation. This usually includes characteristics such as generation time, cell culture complexity, accessibility, genetic manipulation, conservation of mechanisms, expression level, extracellular expression, post-translational modification & processing, scale-up and potential economic benefit.