Genome sizes of all 19 Araucaria species are correlated with their geographical distribution

Abstract

Nuclear genome size was determined to investigate the relationships between all 19 species of Araucaria de Jussieu. Species from the two other genera of Araucariaceae, Wollemia and Agathis, were also studied. The genome size of 17 out of the 19 species of Araucaria are reported here for the first time. All Araucariaceae have the same chromosome number 2n = 26. However, the nuclear DNA contents (2C value) for Araucaria range from 31.3 to 45.4 pg. There is a good correlation between genome size and division in sections, and geographical distribution. The two species from South America have 44.7 and 45.4 pg, the two species from Australia have 35.7 and 44.4 pg and the two species from New Guinea 34.7 and 40.4 pg. All 13 species of New Caledonia and the one from Norfolk Island have a similar, if not identical, amount of nuclear DNA of, on average, 31.9 pg. This corroborates the identical DNA rbcL sequences found for the New Caledonian araucarias. It suggests that the species from New Caledonia diversified more recently and it questions their status as separate species. Compared with this 31.9 pg a strong increase seems to have occurred in the genome size of the "mainland" araucarias. Genome sizes are evaluated and compared with available taxonomic treatments and extant geographic spreading. The nuclear DNA contents found within the sections are close, making it possible to assign an unknown plant to a section. A difference of 1 pg, which amounts to a difference of 978 Mbp, far exceeds a single character. Nuclear DNA content, as measured by flow cytometry, may conveniently be used to produce systematic data. It is applicable even with young plants or seeds for monitoring the trade in endangered species. © 2012 The Author(s).