Droplet digital PCR (ddPCR) is based on the isolated amplification of thousands of individual DNA molecules simultaneously, with each molecule compartmentalized in a droplet. The presence of amplified product in each droplet is indicated by a fluorescent signal, and the proportion of positive droplets allows the precise quantification of a given sequence. In this chapter we briefly outline the basis of ddPCR, and describe two different applications using the Bio-Rad QX200 system: genotyping copy number variation and quantification of Illumina sequencing libraries.
CITATION STYLE
Vossen, R. H. A. M., & White, S. J. (2017). Quantitative DNA analysis using droplet digital PCR. In Methods in Molecular Biology (Vol. 1492, pp. 167–177). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6442-0_11
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