Successful production of hairy root of Valeriana sisymbriifolium by Agrobacterium rhizogenes

Abstract

The successful induction and proliferation of hairy-roots of Valeriana sisymbriifolium explants through the transferece of the rolA gene by Agrobacterium rhizogenes (strain 15834) is reported. Hypocotyls, petiole and leaf explants (0.5-2 cm) were cut from the young plants after sprouting of the sterilized seeds on hormone-free Murashige-Skoog basal medium. The addition of 100 μM acetosyringone to YMB growth medium for Agrobacterium rhizogenes was shown to enhance the inducing ability of the bacterium. Successful and stable transfer of rolA gene was illustrated by PCR using both forward and reverse primers of the gene. © 2008 Asian Network for Scientific Information.