Abstract
A polymerase chain reaction (PCR) in water droplets with water-in-oil emulsion (emulsion PCR) facilitates parallel amplification of a single-molecule template. The amplified DNA can be immobilized onto microbeads bound to primer DNA. The product, termed a "bead library", has various applications such as nextgeneration sequencing (NGS) and the directed evolution of various functional biomolecules. Here, we describe a method for genomic library construction on microbeads using emulsion PCR.
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Kojima, T., Zhu, B., & Nakano, H. (2015). Construction of a DNA library on microbeads using whole genome amplification. In Whole Genome Amplification: Methods and Protocols (pp. 87–100). Springer New York. https://doi.org/10.1007/978-1-4939-2990-0_6
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