Isolation and identification of profenofos pesticide degrading bacterium from soil sample of Bedugul, Indonesia

Abstract

Climate change might change pest populations and in certain circumstances can increase the use of pesticides and the residue on the soil also increases. To reduce the negative impact of the residue, pesticide degrading bacteria are needed. This study aimed to isolate and identify profenofos-degrading bacterial strains from the soil that has been contaminated by profenofos pesticides for many years. The bacterial strain was isolated from a long term profenofos exposed soil by an enrichment technique, and its ability to degrade profenofos was determined using gas chromatography. The results were obtained 8 isolates from 9 sampling points. Isolates have different abilities to degrade profenofos especially isolated BS-06 had the highest degradation rate of profenofos was identified as Pseudomonas luteola according to its physiological and biochemical properties (API 20E system). The isolate has a rod shape, motile, Gram-negative, and has an optimum pH in degrading profenofos at 7. The degradation of profenofos was examined using a medium treated with 100 mg/L profenofos, which resulted in a higher degradation rate than the control without inoculation. In a mineral salt medium (MSPY), removal in the level of profenofos of 92.72% was obtained within 54 h of incubation.