Novel multiresidue method for the determination of eight trichothecene mycotoxins in pollen samples using QuEChERS-based GC-MS/MS

Abstract

A simple multi-residue method based on QuEChERS-sample preparation was developed for the determination of eight trichothecene mycotoxins (nivalenol, fusarenon-x, diacetoxyscirpenol, 3-acetyl-deoxynivalenol, neosolaniol, deoxynivalenol, T-2 and HT-2 toxins) in pollen, using gas chromatography coupled with mass spectrometry. The developed method was validated by evaluating the following parameters: linearity, limit of quantification, matrix effect and precision of measurements. All mycotoxins showed good linearity. The method gave good recoveries (91-104%) with RSDs > 11% for most of the analites (6 out of 8). The limits of quantification varied from 1 to 4μg·kg-1.