This protocol describes how to sample and preserve microbial water column samples from rivers that can be used for 16S or 18S metabarcoding studies or shotgun sequencing. It further describes how to extract the DNA for sequencing and how to prepare raw Illumina MiSeq amplicon data and analyze it in the R environment.
CITATION STYLE
Lehmann, K. (2016). Sampling of riverine or marine bacterial communities in remote locations: From field to publication. In Methods in Molecular Biology (Vol. 1452). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3774-5_1
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