In vitro suppression of CD4 + T-Cell responses by murine and human myeloid-derived suppressor cells

Abstract

Myeloid-derived suppressor cells (MDSCs) are myeloid precursors of macrophages, dendritic cells, and neutrophils with a prominent role in the regulation of immune responses in cancer, infection, and autoimmunity. Herein, we describe a protocol for the isolation of murine and human MDSCs and the assessment of their ability to suppress CD4 + T-cell responses in vitro.