We have carried out chemical shift correlation experiments with symmetry-based mixing sequences at high MAS frequencies and examined different strategies to simultaneously acquire 3D correlation spectra that are commonly required in the structural studies of proteins. The potential of numerically optimised symmetry-based mixing sequences and the simultaneous recording of chemical shift correlation spectra such as: 3D NCAC and 3D NHH with dual receivers, 3D NC′C and 3D C′NCA with sequential 13C acquisitions, 3D NHH and 3D NC′H with sequential 1H acquisitions and 3D CANH and 3D C'NH with broadband 13C- 15N mixing are demonstrated using microcrystalline samples of the β1 immunoglobulin binding domain of protein G (GB1) and the chicken α-spectrin SH3 domain. © 2012 Springer Science+Business Media Dordrecht.
CITATION STYLE
Bellstedt, P., Herbst, C., Häfner, S., Leppert, J., Görlach, M., & Ramachandran, R. (2012). Solid state NMR of proteins at high MAS frequencies: Symmetry-based mixing and simultaneous acquisition of chemical shift correlation spectra. Journal of Biomolecular NMR, 54(4), 325–335. https://doi.org/10.1007/s10858-012-9680-z
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