Isolation and characterization of hydrogenase from Spirulina platensis

Abstract

Hydrogenase fractions were obtained from cultured S. platensis after grinding by centrifugation of 3000-140,000g and discarding the sediments; activity of the enzyme fraction was detd. after column chromatog. purifn. and incubation in the presence of Me viologen and Na dithionite. H evolution activity increased in all fractions at 40,000g centrifugation and then remained const. at 140,000g. In the presence of light, H-evolution activity increased 40% compared to the activity in the dark. One fraction from the cells (fraction II) contained some factor(s) which increased H evolution in the presence of enzyme in the light but had no effect in the dark. on SciFinder(R)