Trichoderma asperellum isolated from salinity soil using rice straw waste as biocontrol agent for cowpea plant pathogens

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Abstract

This study discusses isolation and identification new fungal isolate from salinity soil for controlling soil borne diseases. Among sixteen fungal, a potent isolate coded SRBP_ZSHSG1 was isolated from Sugar beet rhizospher samples collected from Al-Hosainia localities-El-Sharkia-Egypt. Traditional methods consistent with phylogenetic analysis of 18S rRNA sequences showed SRBP_ZSHSG1 has 100% similarity with Trichoderma strains and the most closest is Trichoderma asperellum. Thus, it proposed name Trichoderma asperellum SRBP_ZSHSG1 (ID: KP336489). Results proved SRBP_ZSHSG1 followed by T. roseum and Chaetomium globosum were highly inhibitors to the tested pathogens. These results were confirmed by field experiments. SRBP_ZSHSG1 was able to grow on rice straw (biostraw) and produce most active compounds. The biostaw extract was the most effective bioagent and recorded highest reduction in pathogen numbers. GC/MS analysis of ethyl acetate extract revealed the presence of 9 compounds. These compounds were determined 4 volatile alcohols (1-4) and fatty acid esters (5-9).

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APA

Hamed, E. R., Awad, H. M., Ghazi, E. A., El-Gamal, N. G., & Shehata, H. S. (2015). Trichoderma asperellum isolated from salinity soil using rice straw waste as biocontrol agent for cowpea plant pathogens. Journal of Applied Pharmaceutical Science, 5, 91–98. https://doi.org/10.7324/JAPS.2015.58.S15

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