Effect of chronic administration of date palm seeds extract on some biochemical parameters, oxidative status and caspase-3 expression in female albino rats

Abstract

Date palm seeds have been debated for their beneficial effects on body health. Present study discussed the influences of chronic exposure palm date seeds extract on hepato-renal integrity and feed efficiency. Twenty one Albino female rats were randomly splitted into 3 groups. Group I, is the control and were given oral propylene glycol 10%. Group II, were given palm date seeds extract (200 mg/kg body weight) dissolved in 10% propylene glycol. Group III, were given (400 mg/kg body weight) palm date seeds extract dissolved in 10% propylene glycol. Treatments were given by intra gastric route daily for 60 days. Body weight, weight gain, feed efficiency ratio (FER) were determined. Serum liver and kidney functions tests were detected. Serum super oxide dismutase (SOD) and glutathione peroxidase (GPx) activities were estimated. Caspase-3 protein expressions in kidney and liver were detected by immunohistochemistry (IHC). Results revealed improved FER in group II and III than control. Reduction in serum liver and kidney enzymes were evident in group II and III with improvement in albumin and total protein levels. Caspase-3 IHC in liver and kidney exhibited significant (P<0.05) reduction in palm date seeds extract treated groups especially in group III. Palm date seeds extract, seemed to be safe after chronic administration and has beneficial influences on liver and kidney. The beneficial effects of palm date seeds extract seemed due to its antioxidant effect that reduced hepato-renal caspase-3 protein expression.