Antioxidant Activity of 70% Ethanol Extract Combination of Kemangi Leaf (Ocimum Americanum Linn) and Binahong Leaf (Anredera cordifolia (Ten.) Steenis) Using DPPH

Abstract

Basil and binahong leaf are plants that can be used as medicine. Basil leaf contain alkaloids, flavonoids, saponins and tannins. Binahong leaf contain alkaloids, flavonoid and tannins. These compounds have the ability as an antioxidant to inhibit free radicals. The purpose of this study was to determine the antioxidant activity of a combination of basil leaf extract and binahong leaf. The method used in testing the antioxidant activity is the DPPH free radical scavenging method (1,1-diphenyl-2-pikrihidrazil). Simplicia powder of basil leaf and binahong leaf were extracted by maceration using 70% ethanol solvent. Each extract and extract combination was tested for antioxidant activity against DPPH by measuring the absorbance of DPPH at a wavelength of 516.5 nm. The results showed that the IC50 value for ethanol extract of basil leaf was 42.33 ppm, ethanol extract of binahong leaf was 51.57 ppm, and the IC50 value from each of the combination of ethanol extract of basil leaf and binahong leaf at a ratio of 1:1; 1:2 ; 2;1 was 59.71 ppm ; 77.18 ppm ; 24.33 ppm. Based on the results of the study it can be concluded that the antioxidant activity of the combination of ethanol extract of basil and binahong leaf is very strong compared to the singular both. The combination of ethanol extract of basil leaf and binahong leaf in a ratio of 2:1 has very strong antioxidant activity.