High-efficiency siRNA-based gene knockdown in human embryonic stem cells

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Abstract

Loss-of-function studies in human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) via nonviral approaches have been largely unsuccessful. Here we report a simple and cost-effective method for high-efficiency delivery of plasmids and siRNAs into hESCs and iPSCs. Using this method for siRNA delivery, we achieve >90% reduction in the expression of the stem cell factors Oct4 and Lin28, and observe cell morphological and staining pattern changes, characteristics of hESC differentiation, as a result of Oct4 knockdown. Published by Cold Spring Harbor Laboratory Press. Copyright © 2010 RNA Society.

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Ma, Y., Jin, J., Dong, C., Cheng, E. C., Lin, H., Huang, Y., & Qiu, C. (2010). High-efficiency siRNA-based gene knockdown in human embryonic stem cells. RNA, 16(12), 2564–2569. https://doi.org/10.1261/rna.2350710

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