Isolation of Ectomycorrhizal Fungi : Methods and Techniques

Abstract

This chapter describes various techniques for isolation of ectomycorrhizal fungi. The earlier stages in development of EM in roots can be detected by flurorescence and confocal microscopy. EM fungi can be isolated from roots, solerotia, rhizomosphs and sexual spores i.e. basidiospores by growing on synthetic agar media. The EM fungi are maintained on nutrient agar plates, sterile distilled water or freeze drying of vegetative mycelium.