Multicenter measurements of myelin water fraction and geometric mean T 2: Intra- and intersite reproducibility

Abstract

Purpose: To assess the reproducibility of myelin water fraction (MWF) and geometric mean T2 (GMT2), which are in vivo markers of pathological changes underlying disability and progression in diseases such as multiple sclerosis. Materials and Methods: Five healthy volunteers were scanned twice within 24 hours at six different sites using the same manufacturer's 3T magnetic resonance (MR) system. T2 distributions were produced by fitting multiecho 3D T2 data using non-negative least squares, with stimulated echo correction. MWF, the fraction of signal with T2 between 15 and 40 msec to the entire signal, and GMT2, the mean T2 on a logarithmic scale from T2 between 40 and 200 msec, were examined in white matter. Results: Intrasite coefficients of variation (COVs) were low (mean 3.99% for MWF and 0.51% for GMT2), as were intersite COVs (mean 4.68% for MWF, 0.31% for GMT2). Scan-rescan intraclass correlation coefficients (ICCs) (0.76 for MWF and 0.93 for GMT 2) and Bland-Altman plots indicated good agreement between single site scans. Intersite ICCs were relatively high (0.69 for MWF and 0.92 for GMT2), revealing good intersite reliability. Conclusion: MWF and GMT2 measures are reproducible between scans and across sites with an equivalent MR scanner and sequence protocol. Multicenter clinical trials using quantitative T2 relaxation are feasible. © 2013 Wiley Periodicals, Inc.