Structural studies on O‐specific polysaccharides of lipopolysaccharides from Yersinia enterocolitica serovars O:1,2a,3, O:2a,2b,3 and O:3

Abstract

Lipopolysaccharides from Yersinia enterocolitica serovars O:1,2a,3, O:2a,2b,3 and O:3 have been isolated and characterized. 6‐Deoxy‐L‐altrose residues were shown to be the main constituents of lipopolysaccharides isolated in addition to residues of L‐rhamnose, D‐glucose, D‐galactose, 2‐acetamido‐2‐deoxy‐D‐glucose, 2‐acet‐amido‐2‐deoxy‐D‐galactose, D‐glycero‐D‐manno‐heptose and L‐glycero‐D‐manno‐heptose, 3‐deoxy‐D‐manno‐octulosonic acid being minor components of sugar chains. Mild hydrolysis of lipopolysaccharides with acetic acid furnished O‐specific polysaccharides, which are composed of 6‐deoxy‐L‐altrose. Using 13C‐NMR spectroscopy and methylation data, the structural features of backbones have been elucidated as follows: →2)‐6d‐L‐Altp(β1→2)‐6d‐L‐Altp(β1→3)‐6d‐L‐Altp)(β1→ for serovars O:1,2a,3 and O:2a,2b,3; →2)‐6d‐L‐Altp(β1→ for serovar O:3. In addition, O‐polysaccharide of serovar O:2a,2b,3 was found to contain an O‐acetyl group at the C‐3 position of some 1,2‐linked sugar residues. Copyright © 1985, Wiley Blackwell. All rights reserved