Endothelial sodding of the Permaflow prosthetic coronary artery bypass conduit

3Citations
Citations of this article
9Readers
Mendeley users who have this article in their library.

Abstract

Background. Experiments were designed to determine the feasibility of sodding an endothelial monolayer within the lumen of a prosthetic conduit applied to the canine coronary circulation. Methods. Autologous endothelial cells were sodded onto the luminal surface of the Permaflow conduit and immediately implanted to bypass the left circumflex coronary artery in adult mongrel dogs (n = 9). Unsodded Permaflow conduits were implanted as controls (n = 8). At 3 weeks, grafts were explanted and examined by scanning electron microscopy and immunostained for canine von Willebrand factor. Results. Sodded grafts contained a confluent endothelial cell layer devoid of adherent thrombus or platelets and stained positively for canine von Willebrand factor. Unsodded grafts contained no endothelium and retained adherent platelets, collagen, and fibrin. Effluent from sodded grafts stimulated with calcium ionophore A23187 caused a significantly greater relaxation of its bioassay ring than effluent from unsodded grafts (60% ± 21% versus 12% ± 5%; n = 8, p < 0.03). Conclusions. Sodding of endothelial cells onto a Permaflow coronary artery bypass graft results in a confluent, viable, nonthrombogenic, endothelial monolayer and releases vasodilator substances in response to calcium ionophore A23187. Endothelial sodding may optimize prosthetic grafts.

Cite

CITATION STYLE

APA

Phillips, M. R., Yamaguchi, H., Miller, V. M., Williams, S., Morris, J. J., & Schaff, H. V. (1998). Endothelial sodding of the Permaflow prosthetic coronary artery bypass conduit. In Annals of Thoracic Surgery (Vol. 66, pp. 1191–1197). https://doi.org/10.1016/S0003-4975(98)00780-2

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free