Analysis by enzyme-linked immunosorbent assay and PCR of human liver abscess aspirates from patients in China for Entamoeba histolytica

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Abstract

Aspirates of liver abscesses were analyzed for Entamoeba histolytica. PCR detected a gene encoding a 30-kDa protein in all samples but detected the ribosomal DNA gene in only 14 (33.3%) samples. Enzyme-linked immunosorbent assay detected antigen in 41 (97.6%) samples. PCR analysis of a strain- specific antigen (SSG) revealed that abscesses were caused by various strains.

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Zengzhu, G., Bracha, R., Nuchamowitz, Y., Cheng-I, W., & Mirelman, D. (1999). Analysis by enzyme-linked immunosorbent assay and PCR of human liver abscess aspirates from patients in China for Entamoeba histolytica. Journal of Clinical Microbiology, 37(9), 3034–3036. https://doi.org/10.1128/jcm.37.9.3034-3036.1999

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