Fluorescent, bioluminescent, and optogenetic approaches to study excitable physiology in the single cardiomyocyte

33Citations
Citations of this article
71Readers
Mendeley users who have this article in their library.

Abstract

This review briefly summarizes the single cell application of classical chemical dyes used to visualize cardiomyocyte physiology and their undesirable toxicities which have the potential to confound experimental observations. We will discuss, in detail, the more recent iterative development of fluorescent and bioluminescent protein-based indicators and their emerging application to cardiomyocytes. We will discuss the integration of optical control strategies (optogenetics) to augment the standard imaging approach. This will be done in the context of potential applications, and barriers, of these technologies to disease modelling, drug toxicity, and drug discovery efforts at the single-cell scale.

Cite

CITATION STYLE

APA

Broyles, C. N., Robinson, P., & Daniels, M. J. (2018, June 1). Fluorescent, bioluminescent, and optogenetic approaches to study excitable physiology in the single cardiomyocyte. Cells. MDPI. https://doi.org/10.3390/cells7060051

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free