Fluorescence polarization immunoassay. II. Analyzer for rapid, precise measurement of fluorescence polarization with use of disposable cuvettes

Abstract

This instrument, developed for quantitating fluorescence polarization immunoassays, automatically measures both polarization components and computes a polarization value corrected for background and optical bias. An electronically switched liquid crystal provides a nonmechanical means of rotating the plane of polarization in the excitation optics. The electronic design features digital integration and microprocessor controlled functions. Readings are made in disposable 12 x 75 mm round culture tubes, in less than 10 s. Results are precise to 0.001 polarization unit, linear from 10-7 to 10-10 mol of fluorescein per liter, and correlate well with those obtained with other 'high-performance' instrumentation.