Fluorescent cytoplasm and Heinz bodies of hemoglobin Koln erythrocytes: Evidence for intracellular heme catabolism

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Abstract

Hb Koln, one of the common mutant hemoglobins responsible for unstable hemoglobin disease, was found to be degraded to a fluorescent yellow pigment (FYP) in circulating erythrocytes. FYP is responsible for a strong green fluorescence observed in the cytoplasm and is particularly abundant in the Heinz bodies of Koln RBC. Front face fluorometry and fluorescence microscopy showed that Heinz bodies emit 10% to 20% of the fluorescence of RBCs. Hb-free FYP was obtained by means of a cellulose column separation of the cytoplasm or from a precipitate formed during the incubation of Koln RBC cytoplasm at 50 °C. The absorption and emission spectra of FYP are consistent with those of dipyrroles.

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Eisinger, J., Flores, J., Tyson, J. A., & Shohet, S. B. (1985). Fluorescent cytoplasm and Heinz bodies of hemoglobin Koln erythrocytes: Evidence for intracellular heme catabolism. Blood, 65(4), 886–893. https://doi.org/10.1182/blood.v65.4.886.886

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