Certain nanoparticles have been shown to accumulate within lysosome and hence may cause lysosomal pathologies such as phospholipidosis, lysosomal overload, and autophagy. This chapter describes a method for evaluation of lysosomal activity in porcine kidney cells (LLC-PK1) after exposure to nanoparticles. This method uses the accumulation of a cationic fluorescent dye (LysoTracker Red) in acidic cellular compartments as an indicator of total lysosome content. The lysotracker signal is normalized to the signal from a thiol-reactive dye which is proportional to the total number of viable cells.
CITATION STYLE
Neun, B. W., & Stern, S. T. (2011). Monitoring Lysosomal Activity in Nanoparticle-Treated Cells. In Methods in Molecular Biology (Vol. 697, pp. 207–212). Humana Press Inc. https://doi.org/10.1007/978-1-60327-198-1_22
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