Recently, there have been several successful cases of introducing catalytic activity into proteins. One method that has been used successfully to achieve this is the theozyme placement and enzyme design algorithms implemented in Rosetta Molecular Modeling Suite. Here, we illustrate how to use this software to recapitulate the placement of catalytic residues and ligand into a protein using a theozyme, protein scaffold, and catalytic constraints as input.
CITATION STYLE
Bertolani, S. J., Carlin, D. A., & Siegel, J. B. (2016). Computational introduction of catalytic activity into proteins. In Methods in Molecular Biology (Vol. 1414, pp. 213–231). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3569-7_13
Mendeley helps you to discover research relevant for your work.