Fibroin extracted from silkworm cocoon silk provides an intriguing and potentially important biomaterial for corneal reconstruction. In this chapter we outline our methods for producing a composite of two fibroin-based materials that support the cocultivation of human limbal epithelial (HLE) cells and human limbal stromal (HLS) cells. The resulting tissue substitute consists of a stratified epithelium overlying a three-dimensional arrangement of extracellular matrix components (principally "degummed" fibroin fibers) and mesenchymal stromal cells. This tissue substitute is currently being evaluated as a tool for reconstructing the corneal limbus and corneal epithelium. © Springer Science+Business Media New York 2013.
CITATION STYLE
Bray, L. J., George, K. A., Suzuki, S., Chirila, T. V., & Harkin, D. G. (2013). Fabrication of a Corneal-Limbal Tissue Substitute Using Silk Fibroin. Methods in Molecular Biology, 1014, 165–178. https://doi.org/10.1007/978-1-62703-432-6_11
Mendeley helps you to discover research relevant for your work.