Long noncoding RNA and mRNA expression profiles in the thyroid gland of two phenotypically extreme pig breeds using ribo-zero RNA sequencing

29Citations
Citations of this article
17Readers
Mendeley users who have this article in their library.

Abstract

The thyroid gland is an important endocrine organ modulating development, growth, and metabolism, mainly by controlling the synthesis and secretion of thyroid hormones (THs). However, little is known about the pig thyroid transcriptome. Long non-coding RNAs (lncRNAs) regulate gene expression and play critical roles in many cellular processes. Yorkshire pigs have a higher growth rate but lower fat deposition than that of Jinhua pigs, and thus, these species are ideal models for studying growth and lipid metabolism. This study revealed higher levels of THs in the serum of Yorkshire pigs than in the serum of Jinhua pigs. By using Ribo-zero RNA sequencing—which can capture both polyA and non-polyA transcripts—the thyroid transcriptome of both breeds were analyzed and 22,435 known mRNAs were found to be expressed in the pig thyroid. In addition, 1189 novel mRNAs and 1018 candidate lncRNA transcripts were detected. Multiple TH-synthesis-related genes were identified among the 455 differentially-expressed known mRNAs, 37 novel mRNAs, and 52 lncRNA transcripts. Bioinformatics analysis revealed that differentially-expressed genes were enriched in the microtubule-based process, which contributes to THs secretion. Moreover, integrating analysis predicted 13 potential lncRNA-mRNA gene pairs. These data expanded the repertoire of porcine lncRNAs and mRNAs and contribute to understanding the possible molecular mechanisms involved in animal growth and lipid metabolism.

References Powered by Scopus

Analysis of relative gene expression data using real-time quantitative PCR and the 2<sup>-ΔΔC</sup>T method

150005Citations
N/AReaders
Get full text

Fast gapped-read alignment with Bowtie 2

36312Citations
N/AReaders
Get full text

Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation

12228Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Genome-wide analysis of mRNAs and lncRNAs of intramuscular fat related to lipid metabolism in two pig breeds

45Citations
N/AReaders
Get full text

Review: Long non-coding RNA in livestock

43Citations
N/AReaders
Get full text

Comparative analyses of long non-coding RNA in lean and obese pig

42Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Shen, Y., Mao, H., Huang, M., Chen, L., Chen, J., Cai, Z., … Xu, N. (2016). Long noncoding RNA and mRNA expression profiles in the thyroid gland of two phenotypically extreme pig breeds using ribo-zero RNA sequencing. Genes, 7(7). https://doi.org/10.3390/genes7070034

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 6

50%

Researcher 5

42%

Professor / Associate Prof. 1

8%

Readers' Discipline

Tooltip

Biochemistry, Genetics and Molecular Bi... 5

42%

Agricultural and Biological Sciences 4

33%

Veterinary Science and Veterinary Medic... 2

17%

Neuroscience 1

8%

Article Metrics

Tooltip
Social Media
Shares, Likes & Comments: 1

Save time finding and organizing research with Mendeley

Sign up for free