Quantitative and spatial image analysis of tumor and draining lymph nodes using immunohistochemistry and high-resolution multispectral imaging to predict metastasis

Abstract

Immunohistochemistry is an essential tool for clinical and translational research laboratories. It is mostly used as a qualitative measure of morphology and cell types within tissue. We have developed a high-resolution multispectral imaging method to expand the uses of immunohistochemistry by making it quantitative. In this chapter we describe the technology, both hardware and software, that we use for this method and give examples of applications. © Springer Science+Business Media New York 2014.