The emergence of the clustered, regularly interspaced, short palindromic repeat (CRISPR) technology provides tools for researchers to modify genomes in a specific and efficient manner. The Type II CRISPR-Cas9 system enables gene editing by directed DNA cleavage followed by either non-homologous end joining (NHEJ) or homology-directed repair (HDR). Here, we described the use of the Type II CRISPR-Cas9 system in detail from designing the guides to analyzing the desired gene disruption events.
CITATION STYLE
Hu, N., & Malek, S. N. (2019). Gene Disruption Using CRISPR-Cas9 Technology. In Methods in Molecular Biology (Vol. 1881, pp. 201–209). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8876-1_16
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