MiR-3666 suppresses cellular proliferation and invasion in colorectal cancer by targeting SATB2

Abstract

MicroRNA-3666 (miR-3666) acts as a tumor suppressor in cervical cancer, non-small cell lung cancer and thyroid carcinoma; however, the function of miR-3666 in colorectal cancer (CRC) remains largely unknown. In the present study, was demonstrated that miR-3666 was significantly downregulated in CRC tissues compared with in adjacent normal tissues by reverse transcription-quantitative polymerase chain reaction. Additionally, miR-3666 may serve as a prognostic biomarker for patients with CRC. Via functional experiments, the present study reported that miR-3666 overexpression significantly inhibited the proliferation, migration and invasion of CRC cells as determined by Cell Counting Kit-8 and Transwell assays, and vice versa. In addition, miR-3666 was reported to directly target special AT-rich sequence binding protein 2 (SATB2) in CRC cells; overexpression of miR-3666 significantly suppressed the expression of SATB2 in CRC cells as determined by western blotting. Furthermore, an inverse correlation was observed between the expression levels of miR-3666 and SATB2 in CRC tissues. Restoration of SATB1 expression significantly reversed the effects of miR-3666 mimic on CRC cells. In summary, the results of the present study indicated that miR-3666 may serve as a tumor suppressor in CRC by targeting SATB2.