SNP microarray-based 24 chromosome aneuploidy screening is significantly more consistent than FISH

Abstract

Many studies estimate that chromosomal mosaicism within the cleavage-stage human embryo is high. However, comparison of two uniquemethods of aneuploidy screening of blastomeres within the same embryo has not been conducted andmay indicatewhethermosaicism has been overestimated due to technical inconsistency rather than the biological phenomena. The present study investigates the prevalence of chromosomal abnormality and mosaicism found with two different single cell aneuploidy screening techniques. Thirteen arrested cleavage-stage embryos were studied. Each was biopsied into individual cells (n = 160). The cells from each embryo were randomized into two groups. Those destined for FISH-based aneuploidy screening (n = 75) were fixed, one cell per slide. Cells for SNP microarray-based aneuploidy screening (n = 85) were put into individual tubes. Microarray was significantly more reliable (96%) than FISH (83%) for providing an interpretable result (P = 0.004). Markedly different results were obtained when comparing microarray and FISH results from individual embryos. Mosaicism was significantly less commonly observed bymicroarray (31%) than by FISH (100%) (P = 0.0005). Although FISH evaluated fewer chromosomes per cell and fewer cells per embryo, FISH still displayed significantly more unique genetic diagnoses per embryo (3.2±0.2) than microarray (1.3±0.2) (P< 0.0001). This is the first prospective, randomized, blinded and paired comparison between microarray and FISH-based aneuploidy screening. SNPmicroarray-based 24 chromosome aneuploidy screening provides more complete and consistent results than FISH. These results also suggest that FISHtechnology may overestimate the contribution of mitotic error to the origin of aneuploidy at the cleavage stage of human embryogenesis. © The Author 2010. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology.