Analytical Method Development and Validation of Dimethoate Pesticide using HPLC Method

Abstract

Volume 2 • Issue 2 • 1000127 Biochem Anal Biochem ISSN:2161-1009 Biochem, an open access journal Overview Dimethoate (Figure 1) is a widely used organophosphate insecti-cide used to kill insects on contact. It was patented and introduced in the 1950s by American Cyanamid. Like other organophosphates, di-methoate is an anti-cholinesterase which disables cholinesterase, an en-zyme essential for central nervous system function [1]. The literature survey, it was found that dimethoate estimated by analytical methods such reversed-phase high performance liquid chromatographic HPLC method [2] and some spectrophotometric methods like mass spectro-photometry. The HPLC method has its relative merits but the majority of them are carried out at elevated temperatures, time consuming, use relatively expensive reagents, involve extraction, use of buffer system. In this report, we present one simple, sensitive, cost-effective and accurate method for the determination of dimethoate [3]. Materials and Methods Instrumentation A HPLC equipped with UV detector was used for the present research work. The separation was achieved using Phenomenex luna C18 column 250×4.6. Chemicals and reagents Dimethoate sample was purchased from Sigma-Aldrich Company. All the chemicals are of analytical reagent grade of Merck Pharmaceuticals. HPLC grade water was used to prepare all solutions. Method development Selection and preparation of mobile phase: Various mobile phases were tried in different ratios for selection of mobile phase. The drug Dimethoate was injected with different mobile phases at different ratios with different flow rates till a sharp peak, without any interference peaks containing spectrum was obtained. The mobile phase selected was acetonitrile and water in the ratio 60:40 (v/v).