Corrigendum: The TbD1 Locus Mediates a Hypoxia-Induced Copper Response in Mycobacterium bovis(Front. Microbiol., (2022), 13, (817952), 10.3389/fmicb.2022.817952)

Abstract

In the original article, there was an error. It was stated that sodium pyruvate was added to Mycobacterium bovis AF2122/97 growth media to a final concentration of 10mM, which is not correct; the correct concentration is 40mM. A correction has been made to two sections of the Materials andMethods, Bacterial Strains and Culture Conditions, paragraph one: “Escherichia coli strains that were used for plasmid propagation in MultiSite Gateway cloning (Life Technologies/Invitrogen/Thermo Fisher Scientific, Loughborough, United Kingdom)procedures were grown in LB medium or LB agar plates supplemented with selected antibiotics. Ampicillin (50 μg/ml), zeocin (25 μg/ml), and hygromycin (50 μg/ml) were added as required. BCG Denmark was grown in liquid Middlebrook 7H9 medium (Becton Dickinson, New Jersey, NJ, United States) supplemented with 0.05% Tween 80, 0.2% glycerol, 0.5% bovine serum albumin, 0.2% glucose, and 0.085% NaCl or 7H11 agar plates (Becton Dickinson, New Jersey, United States)supplemented with 0.2% glycerol, 0.5% BSA, 0.2% glucose, and 0.085% NaCl. M. bovis AF2122/97 was grown in the 7H9 and 7H11 media as described above, with 40 mM sodium pyruvate (Sigma- Aldrich, Ireland).When required, kanamycin, hygromycin, or zeocin were added to growth media to a final concentration of 50, 50, or 25 μg/ml, respectively. Standing cultures for RNA extraction were grown in 30ml of 7H9 in 50ml tubes (Sarstedt) with the caps tightly screwed, without shaking at 37◦C. Rolling cultures for RNA extraction were grown in 30 ml of 7H9 in 850 cm2 roller bottles (Cellmaster), rolling at 2–3 rpm at 37◦C. Sauton’s medium was prepared using 4 g L-asparagine, 2 g citric acid, 0.5 g KH2PO4, 0.5 g MgSO4, 0.05 g ferric ammonium citrate, 0.1 ml of 0.01% ZnSO4, 60 ml glycerol, 2.5 ml of 20% Tween 80 in 900 ml deionized water and adjust pH to 7.0 with 1 M NaOH, adding 40 mM sodium pyruvate for M. bovis AF2122/97. The strains used in this study are listed in Supplementary Table 1.” In addition, a correction has been made to the Materials and Methods, Drop Assays and Copper Challenge, paragraph one:.